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Catalog # Kit Name
RIF0600M Epstein-Barr Virus (EBV) VCA IgM Recombinant IFA (RIFA®)
Recombinant immunofluorescent antibody ( RIFA®) test for the detection and semi-quantitation of human serum IgM antibodies to Epstein-Barr viral capsid antigen (VCA). Each kit contains ten 12-well slides prepared with recombinant EBV VCA antigen, IgM pretreatment diluent, IgM RIFA® conjugate, and positive and negative controls.
 
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EBV VCA IgM (RIFA®)

Slide 1 Slide 2
IgM Positive Sample IgM Negative Sample
[Picture of EBV rViral slide] [Picture of EBV rViral slide]
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EBV VCA IgM Recombinant IFA Slide Schematic
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Frequently Asked Questions (FAQ)
Q: What does RIFA stand for?
A: It is a trademark for Recombinant Immunofluorescent Assay. It is used in the VCA IgM IFA kit.

Q: Why is the conjugate so dark in this kit?
A: It has a high fluorescein/protein ratio and additional counterstain has been added. 

Q: There doesn’t seem to be much whole cell fluorescence with this antigen why is that?
A: The recombinant seems to give a bulls-eye type fluorescent pattern. The fluorescence around the edge of the cell is more intense with the center having a dimmer fluorescence.
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Epstein-Barr Virus (EBV) VCA IgM Recombinant IFA (RIFA<sup>®</sup>)
       
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